EFFECT OF ALOE VERA JUICE ON TOTAL BLOOD COUNT AGAINST TOXICITY INDUCED BY ETHIONAMIDE AND PARA AMINO SALICYLIC ACID IN SPRAGUE-DAWLEY RATS

Fresh Aloe vera plant leaves were brought from botanical garden and sample was identified and brought to the laboratory in the Department of Zoology, Patkar-Varde College, Goregaon (W), Mumbai. 50 grams of leaves were then grounded with 50ml of distilled water in sterilized pestle and mortar. The yield will be calculated based on weight of the extract compared to the weight of the pulp of the leaves. Forty-eight (48) Sprague- dawley rats (average weight 150 – 250 g) of either sex were used for the experiment. The ETH and PAS drug and Aloe vera juice were given to respective groups daily for 28 days. At the end of the study animals were anesthetized and were sacrificed by cervical decapitation. Blood was collected via cardiac puncture and studied for WBC: White blood cell count, LYM: Lymphocytes, MID: Indicates the combined value of the other types of white blood cells not classified as lymphocytes or granulocytes, NEUT: Neutrophils, RBC: Red blood cell count, HGB: Hemoglobin, HCT: Hematocrit and PLT: The platelet counts by using Operon hematology analyzer. Graph Pad Prism 7 was used for statistical analysis by one way variance (ANOVA). The value (p< 0.05) considered as significant and (P< .00001) is no significant. The present study demonstrated that the anti- tuberculosis drugs ETH and PAS changes the hematological parameters due to the presence of toxic metabolites. The toxic metabolites of the drugs bind to cellular macromolecules and released to form toxic free radicals which in turn it caused tissue damage. Whereas, we observed that after administration of Aloe vera juice independently or in combination with the anti-TB drugs ETH and PAS the hematological parameters found improved towards normalization. Based on the above results it is concluded that the Aloe vera juice have a good bio-enhancer property against toxicity induced by ETH and PAS drugs in Sprague-dawley rats