EFFECT OF AM FUNGI AND DIFFERENT SELENIUM LEVELS ON THE QUALITY OF SALVIA MILTIORRHIZA BGEAbstract
The effects of AM fungi on Salvia miltiorrhiza under different selenium levels was researched using pot technology. The results showed that inoculation of AM fungi significantly increased the plant height, root length, each part weight, chlorophyll, protein and selenium content of Salvia miltiorrhiza under the same selenium level, in addition, MDA and proline contents decreased significantly. It can be seen that AM fungi could promote the growth of Salvia miltiorrhiza, enhance disease resistance and improve the uptake of nutrient elements. The contents of tanshinone IIA in inoculated plants were higher than non inoculated plants. While the selenium concentration was 40μg.ml-1, the content of tanshinone IIA in inoculated plants was highest, that was 0.272%. The growth of Salvia miltiorrhiza, soluble sugar, MDA and the content of tanshinone IIA increased firstly and then decreased, but the contents of inoculated plants were higher than non inoculated plants, they reached the maximum at Se1(20μg.ml-1)~Se2(40 μg.ml-1). From that proper concentration was cooperated with AM fungi on improving the growth and accumulating of medicinal components. Selenium content in Salvia miltiorrhiza Bge was positively correlated with selenium concentrations, but no obvious linear relationship, the distribution was more in the leaf than root. Inoculation of AM fungi on the Salvia miltiorrhiza had the best effect when the selenium concentration was 20~40 μg.ml-1.
L. I. Jianheng *, QIAO Yajun, Qi Junxiang, WU Hanqiu and YANG Xiaoyu
College of Pharmaceutical Sciences, Hebei University, Baoding Hebei, China
22 March, 2015
06 June, 2015
22 September, 2015
01 October, 2015