EFFECT OF AMLODIPINE ON ORAL GLUCOSE INDUCED GLYCEMIC CHANGES IN NORMAL ALBINO RATSAbstract
Objective: To determine the effect of amlodipine on blood glucose levels through oral glucose tolerance test in normoglycemic albino Rats and the magnitude of its effect on basal v/s glucose induced glycemic value compared to control.
Methods: Rats were divided into control and test groups to study the effect of glucose induced glycemic changes in normal rats following oral administration of amlodipine. The control group received 1 ml of distilled water everyday, test group received amlodipine everyday in the dose of 1.5 mg/Kg BW for 3 days.On the third day, 2 hours after drug administration both groups were administered oral glucose in the dose of 0.6 gm/Kg BW. The blood glucose levels were measured at 0, 60 and 150 minutes after glucose administration by rat tail snipping method using ACCUCHEK glucometer.
Results: The mean CBG of Test group is significantly higher(P<0.001) at all times of the glucose challenge i.e. 0, 60, 150 minutes from the time of glucose administration compared to control group. The optimal hyperglycemia was seen at 60 minutes which is 32.76% higher than the control group, followed by 0 minutes (29.41%) and 150 minutes (7.92%).
Conclusion: Amlodipine worsens glycaemic control in normal rats at all hours of glucose challenge. Extending this to human beings, whether with impaired glucose tolerance or overt diabetes mellitus, it is suggested to limit the use of amlodipine to situations unless absolutely necessary since it induces hyperglycaemia even in normoglycaemic rats by a postulated mechanism of inhibition of both basal and glucose induced insulin secretion significantly.
Sushma V. Naidu*, R.N. Suresha, J.C. Huralikuppi, V. Ashwini, A.M. Satish and M. Brahadeesh
Department of Pharmacology, JSS Medical College, (A Constituent College of JSS University, Mysore), SS Nagar, Mysore, Karnataka, India
26 May, 2012
29 July, 2012
29 August, 2012
01 September, 2012