EFFECT OF ETANERCEPT AGAINST MYOCARDIAL ISCHEMIA/REPERFUSION INJURY IN MALE MICEAbstract
Objective: This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model. Materials & methods: Adult male Swiss Albino mice were randomized into 4 equal groups. Group (1) sham group: mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery , Group( 2) control group: mice subjected to regional ischemia for 30 min and reperfusion for 2 hours by ligation of LAD coronary artery ,Group( 3) control vehicle group: same control group except mice given IP normal saline before reperfusion 5 minutes, Group( 4) Etanercept treated group : mice treated with etanercept 5 mg/kg i.p. 5 minuts before reperfusion of ligation of LAD coronary artery. The heart tissue (below the ligation site) was used for measurement of apoptosis (caspase 3 and Bcl-2)and histopathology study. Results: Histologically, all mice in control group showed significant (p<0.05) cardiac injury. Furthermore all mice in control group showed significant (p<0.05) of caspase 3 while significant decrease (p<0.05) of Bcl-2. Etanercept significantly counteract the increase in myocardium level of caspase 3 (P < 0.05), etanercept significantly counteract the decrease (p<0.05) in myocardium level of Bcl-2. Histological analysis revealed that etanercept markedly reduced (P < 0.05) the severity of heart injury in the mice underwent LAD ligation procedure. Conclusion: The etanerceptameliorate myocardial I/R injury in mice via interfering with apoptosis which induced by I/R injury.
Saif M. Hassan
Department of Pharmacology and Therapeutics, College of Medicine, Kufa University, Kufa, Iraq
15 December, 2014
05 February, 2015
22 July, 2015
01 September, 2015