EFFECT OF GERMINATION ON POLYPHENOL CONTENTS AND ANTIOXIDANT ACTIVITY IN MUSTARD SEEDS (BRASSICA NIGRA) GROWN IN ETHIOPIAAbstract
Mustard seeds, in some countries, are used as a traditional medicine for the treatment of snakebite, toothache, and epilepsy, as well as to treat skin diseases because of their high sulphur content. The aim of this study was to investigate the effect of three germination periods on the polyphenol contents and antioxidant activity of mustard seeds. Phenolic compounds were determined by the Folin-Ciocalteau method, while antioxidant activity was determined using standard methods. The results indicated that the total phenol and flavonoid contents of the one, two, and three days germinated mustard seed were increased by 16%, 17%, 24% and 500%, 647%,747%, respectively. Antioxidant activity determined by reducing power assay (for one, two and three days of germinated mustard seed) also increased by 10%, 34%, 51%, respectively. Germination is thus a better technique to enhance the nutritional and pharmaceutical potential of mustard seed and can be used in the development of functional diets.
M. Efrem, R. Negussie, B. Sekwati-Monang, R. I. Kobue-Lekalake, T. Selebatso, M. R. Setlalekgomo and G. D. Haki *
Botswana University of Agriculture and Natural Resources, Gaborone, Botswana.
22 February 2022
26 April 2022
29 April 2022
01 October 2022