EFFECT OF HYDROALCOHOLIC EXTRACT OF SIDA SPINOSA L. ON 2,4,6-TRINITRO-BENZENESULFONIC ACID INDUCED ULCERATIVE COLITIS IN RATSAbstract
The present study aimed to evaluate the effect of hydroalcoholic extract of Sida spinosa L. (HYSS) in colitis induced in rats by intrarectal administration of TNBS by clinical, morphological and biochemical alterations. The HYSS administered at three different concentrations 100, 200 and 400 mg/kg, p.o. and sulfasalazine (50 mg/kg, p.o) as reference standard for 10 days in colitis induced rats. TNBS administration caused induction of colitis resulting in significant reduction in percentage body weight, increased stool consistency score, macroscopic score, colon weight, weight to length ratio, ulcer area, ulcer index etc. It also caused elevation of oxidative stress i.e. increased MDA, MPO level and depleted GSH level. It also resulted in histological changes in colon architecture suggestive of extensive mucosal damage associated with intermittent inflammatory changes and infiltration of inflammatory cells in mucosa and submucosa. HYSS at 200 & 400 mg/kg significantly restores loss of percent body weight, reduced stool consistency score, ameliorate macroscopic changes, histological changes, colon weight to length ratio, ulcer index, reduced MPO, MDA level and restores GSH level when compared to TNBS induction control group. Results of present study indicates the anti-inflammatory and immunomodulatory potential of HYSS to heal TNBS induced colitis in rats.
A. V. Kulkarni * and N. S. Vyawahare
Department of Pharmacology, Dr. D. Y. Patil College of Pharmacy, Savitribai Phule Pune University, Pune, Maharashtra, India.
12 January 2020
09 March 2020
11 March 2020
01 January 2021