EFFECT OF INCENSE SMOKE ON THE BEAS-2B AND EB-3 CELLSAbstract
Usage of incense smoke has aesthetic importance. But, studies have shown that incense smoke has oxides of carbon, nitrogen, sulphur, which has ill effects on human health. Polyphenol analysis showed the presence of phenol-like compounds in the water extracts of the incense. This study was conducted to understand the impact of exposure of cells to these types of smokes by performing viability test using squamous epithelial cells. Different methods followed to confirm the results, and the results of these studies showed that 80% of the squamous epithelial cells survived when exposed to smoke as compared to controls (no smoke treatment). Further studies were extended on the cell lines BEAS-2B (normal lung cells) and cancerous cells EB-3 cells. Results based on the catalase assay and microscopic studies show that incense smokes have a lethal effect on the normal cells while not showing any harmful effects on cancerous cells and these studies reproduced using trypan blue assay.
R. R. Hegdekatte *, N. R. Kumari, A. P. Uppar and P. G. Anantharaju
Department of Biochemistry, Pooja Bhagavat Memorial Mahajana Education Centre, Post Graduate Wing of SBRR Mahajana First Grade College, Mysuru, Karnataka, India.
03 April 2020
30 August 2020
11 September 2020
01 April 2021