EFFECT OF MORCHELLA CONICA AND FOMES FOMENTARIUS EXTRACTS AGAINST THE HUMAN RESPIRATORY SYNCYTIAL VIRUS IN-VITROAbstract
Antiviral properties of methanol and aqueous extracts from Morchella conica and Fomes fomentarius were assessed by colorimetric XTT test against human respiratory syncytial virus (HRSV). HEp-2 cell line was used for the cytotoxic and antiviral effects of the extracts. Non-cytotoxic concentrations of methanol and aqueous extracts of M. conica were tested in 50000 to 195 µg/mL. In contrast, the cytotoxic F. fomentarius methanol and aqueous extracts and Ribavirin were tested at concentrations from 3347-13.1 μg/mL, 4906.5-19.2 μg/mL and 86.60-0.30 μg/mL, respectively. The results showed that aqueous extract of F. fomentarius had strong anti-HRSV activity (EC50 = 358.7 μg/mL, SI = 27.4) which could be compared with ribavirin (EC50 = 15.6 μg/mL, SI = 11.1) used as a positive control against HRSV, while other extracts (M. conica methanol extract EC50 = 28055 μg/mL, SI = >1.8; F. fomentarius methanol extract EC50 = 3000 μg/mL, SI = 2.2) were found to have weak antiviral activity. As a result, it can be said that aqueous extract of F. fomentarius is worthy of further study as an alternative drugs in order to develop anti RSV.
H. H. Doğan *, R. Duman and P. Tuncer
Department of Biology, Science Faculty, Campus, Selcuk University Konya, Turkey.
10 April, 2018
06 June, 2018
14 June, 2018
01 December, 2018