EFFECT OF NUTRIENTS ON LIVER MARKER ENZYMES OF WISTAR RATS

A wide range of compounds are found to be effective against cancer for example Vitamin D₃, lysine, proline, gallate, quercetin, etc.  Glucosinolates from cabbage are secondary metabolites hydrolyzed by the enzyme myrosinase ¹. Their breakdown products such as isothiocyanates, nitriles and thiocyanates, are chemically very reactive, and they are known for their bioactive characteristics, such as anticarcinogenic, fungicidal and bactericidal properties ². The objective of this study was to formulate a nutrient medium containing glucosinolates from cabbage, Vitamin D₃, proline and selenium and study its effect on the SGOT, SGPT and ALP levels. To see the synergistic effect of these nutrients, along with glucosinolates from cabbage two nutrient medium were formulated, NM1 (100 mg/kg BW of rats of Brassica oleracea extract+ 25 mg/kg BW of Vitamin D₃+ 4 µg/kg BW selenium+ 500 mg/kg BW of proline) and NM2 (200 mg/kg BW of rats of  Brassica oleracea extract+ 50 mg/kg BW of Vitamin D₃+ 6 µg/kg BW selenium+ 1000 mg/kg BW of proline) ³. These NM were administered to Wistar albino rats, divided into three groups. Each group housed 5 rats (2 males and 3 females). The effect of administration of NM1 and NM2, to above mentioned rats, once daily for 14 days on the liver markers was investigated. On 15^th day, the rats were sacrificed, liver was surgically removed. Liver sections were analyzed histopathologically. Decrease in SGPT and SGOT levels as compared to normal control was observed. Histology of liver tissue, also corroborated with the biochemical analysis