EFFECT OF PIPER BETLE LEAF EXTRACT AND SYZYGIUM AROMATICUM FLOWER EXTRACT AND THEIR ACTIVE COMPONENT EUGENOL ON THE SURVIVAL OF LUNG ADENOCARCINOMA CELLS (A549)Abstract
Medicinal plants are one of the most important resources for developing new lead therapeutic compounds used in various traditional medicines worldwide for thousands of years due to the presence of natural bioactive components, which help in preventing various human diseases. Piper betle Linn belongs to the Piperaceae family, commonly known as Betel and Paan. Betel leaves possess several bioactivities, and these are used in traditional medicinal systems. Syzygium aromaticum L. (Cloves) is a tree belonging to the Myrtaceae family. Closed flowering buds are used as a spice in food preparations all over the world. Eugenol is an active compound of both plants (Piper betle and Syzygium aromaticum). Hence, we attempted to study the effect of the methanolic extracts of Piper betle and Syzygium aromaticum, along with their active component, eugenol, using an in-vitro approach. The in-vitro cytotoxicity was assessed against lung adenocarcinoma cell lines (A549) using the MTT and SRB assays, and the type of cell death was characterized by differential staining. The results showed that the plant extracts, as well as eugenol, showed good anticancer potential in-vitro.
C. Azhagumeena, P. R. Padma and S. Sumathi *
Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, India.
03 April 2020
14 August 2020
03 November 2020
01 April 2021