EFFECT OF SOLANUM CHRYSOTRICHUM SCHLDL. ON CELL PROLIFERATION IN VITRO

The effect of four extracts of Solanum chrysotrichum on the proliferation of bacteria, yeasts, mouse spleen cells and human transformed cells was determined. Dry leaves grounded were consecutively macerated 24 h with hexane, chloroform, methanol and water. Dilutions of 1, 10 and 100 µg/mL / DMSO were used for the assays with normal mouse spleen cells, DU-145 and MCF-7 cells. The cellular proliferation was evaluated by the sulforhodamine method´s. Antibacterial and antifungal activities were determined by methods of diffusion and dilutions, using concentrations of 10, 15, 20 and 25 mg/mL. The bacteria used were Escherichia coli SOS, Proteus mirabilis NCTC2896, Staphylococcus aureus ATCC6538 and Bacillus subtilis. The yeasts were Candida albicans, Candida tropicalis and Cryptococcus neoformans. The bacteria were cultured in Agar-Mueller-Hinton and the yeasts in Sabouraud Agar and incubated at 37 and 28 ºC, respectively 24 h. Methanol and watery extracts at dosage assayed caused a cellular concentration reduction average of 56 and 59%, on cells DU-145, respectively. The chloroform extract at doses of 100 µg/mL increased to 40%, the concentration of mouse spleen cells, the other extracts had a cytostatic effect. This extract also reduced to 53% concentration of MCF-7 cells at 10 and 1 µg/mL. All concentrations of methanol extract inhibited to E. coli SOS and the concentration of 25 mg/mL also inhibited to C. neoformans. The aqueous extract inhibited the growth of B. subtilis with all concentrations assayed and to the other bacteria and C. neoformans with the 25 dose of mg/mL.