ENCAPSULATION OF CURCUMIN IN SILVER NANOPARTICLE FOR ENHANCEMENT OF ANTICANCER DRUG DELIVERYAbstract
In the proposed work, enhancement of anticancer potential of curcumin extracted from Curcuma longa Linn. by encapsulated in the silver nanoparticle. Curcumin was extracted from Curcuma longa by Soxhlet method. Curcumin is a kind of poly phenols which was widely used because of its medicinal application. Curcumin shows poor solubility and low absorption rate therefore its silver nanoparticles was formed to increase solubility, absorption as well as affectivity against tumor. Various analytical methods and assay such as Particle size, Zeta potential, Atomic Force Microscopy (AFM), Differential Scanning Colorimetric analysis (DSC), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR), Drug Release, Hemolytic toxicity study and In-vitro cytotoxicity study were employed for monitoring the formation of nanoparticles. Presence of silver nanoparticles with an average size of 17.98 nm was revealed by Atomic Force Microscopy and particle size analyzer and zeta potential may obtained -4.68 mV. The result of hemolytic toxicity was demonstrated that Cur-AgNPs shows lesser hemotoxicity in comparison with plain curcumin and Cur-AgNPs were able to release 93.15% curcumin in 12 hrs whereas the plain curcumin was released 93.31% in 4 hrs. The crystalline nature of synthesized nanoparticles was done by X-ray diffraction. The results obtained by in-vitro cytotoxicity study, demonstrated that Cur-AgNPs cytotoxicity induced by curcumin to the tumor cells were dose dependent and had a greater cytotoxic effect on the tumor cells than plain curcumin. This work shows that curcumin encapsulated silver nanoparticles shows potential activity against cancer cell.
S. Garg* and A. Garg
Department of Chemistry and Pharmacy, Rani Durgawati Vishwavidhyalaya, Jabalpur, Madhya Pradesh, India.
15 June, 2017
18 August, 2017
29 August, 2017
01 March, 2018