ENHANCEMENT OF BIOACTIVE CRUDE COMPOUND PRODUCTION BY STATISTICAL OPTIMIZATION OF FERMENTATION PARAMETERS FROM STREPTOMYCES SP. NLKPB45 ISOLATED FROM MARINE SOIL SEDIMENTAbstract
Actino bacterium Streptomyces sp. NLKPB45, which was isolated from mangrove soil sediments of Nellore coastal regions of Andhra Pradesh, India, was used in shake-flask fermentation to explore the different carbon and nitrogen substrates for antimicrobial crude compound. Careful experimentation of ranging each variable in the fermentation medium found that glucose and peptone as preferred carbon and nitrogen sources at 1% (w/v) concentration each, at pH of 7 and 28 °C incubation temperature for 8 days of incubation time. Response surface methodology-based statistical optimization of the process variables with their interconnection was analyzed using a central composite design. A second-order quadratic polynomial equation yielded a complacent fit for experimental with respect to crude compound concentration. Analysis of variance statistics showed that significant-high R2 value for model and adjusted and predicted; R2 values were also shown decent agreement with observed and predicted results. The results have confirmed very good interaction among five process variables at optimized values of 7.51318 pH, 28.734 °C incubation temperature, 8.18409 days incubation time, 1.05185 g/L glucose and 1.0483 g/L peptone yielded 4.5873 g/L of crude compound concentration, which is about 20% increase due to statistical optimization by Streptomyces sp. NLKPB45.
Susha B. Kalyani *, P. S. Krishna and K. Sreenivasulu
IKP-Life science Incubator, Hyderabad, Telangana, India.
15 December 2020
11 March 2021
26 May 2021
01 November 2021