ESTIMATION OF QUERCETIN BY HIGH PERFORMANCE CHROMATOGRAPHY AND ANTIFUNGAL ACTIVITY OF MOSS PHILONOTIS REVOLUTAAbstract
Bryophytes- the small, slow growing group of plants is stockroom of naturally occurring materials. They contain a high number of biologically active compounds, however their use as a natural fungicides is negligible. In the recent years bryophytes has emerged as a potential biopharming tool for production of complex biopharmaceutiticals. In order to evaluate potential use of bryophytes as an antifungal compounds their basic chemical contents and the secondary metabolite profile was determined. Among secondary metabolites flavonoids are a group of polyphenolic compounds, which are widely distributed throughout the plant kingdom. Flavonoids like Rutin and quercetin possess many biochemical effects. An HPLC method was developed for the estimation of quercetin from methanol extract of Philonotis revoluta bryophyte. The results of the phytochemical screening using different extracts revealed the presence of alkaloids, flavonoids, phenols, etc. in the moss plant and investigated against plant pathogenic fungi using different parameters. Results showed significant reduction in pathogenic growth.