EVALUATION OF ACUTE AND SUBCHRONIC TOXICITY OF LAGENARIA SICERARIA AERIAL PARTSAbstract
Lagenaria siceraria is traditionally being used in many countries in the treatment of various diseases including diabetes. The aim of the present study was to evaluate the safety of the methanol extract of L. siceraria aerial parts (MELS) through acute and subchronic toxicity study in mice. For acute toxicity study 500-2000 mg/kg MELS were administered orally and obvious toxic symptoms and mortality was studied upto 72 h. In subchronic study, effect of multiple weekly dosing of 400 mg/kg (one-fifth of the maximum tolerated dose) of MELS was investigated in mice for six weeks and the evaluation was done by the studies of hematological parameters, biochemical estimations of hepatorenal parameters, antioxidant status, and histological observations of the tissue. The extract was found to be well tolerated upto 2g/kg in acute toxicity study. In subchronic toxicity study it showed no significant alteration on any of the parameters, however an improvement in the lipid profile was observed in the treated group of animals. Hence the results suggest that methanol extract of L.siceraria aerial parts is quite safe and can be used in the treatment of the chronic diseases like diabetes without any toxicity.
P. Saha , U. K. Mazumder*, P. K. Haldar , A. Islam and R. B. Suresh Kumar
Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India
26 March, 2011
13 May, 2011
28 May, 2011
01 June, 2011