EVALUATION OF ACUTE TOXICITY OF NEEM ACTIVE CONSTITUENT, NIMBOLIDE AND ITS HEPATOPROTECTIVE ACTIVITY AGAINST ACUTE DOSE OF CARBON TETRACHLORIDE TREATED ALBINO RATSAbstract
Objective: To investigate the neem active constituent nimbolide for the evidence of acute toxicity and its protective effects against carbon tetrachloride (CCl4) induced liver toxicity in rats.
Materials and Methods: Group allotment in hepatoprotective activity study included vehicle, CCl4 (1ml/kg), Silymarin (100 µg/kg/day) + CCl4 and graded doses of nimbolide (100 and 200µg/ kg/ day) + CCl4. On 9th day, blood was obtained for determination of biochemical parameters and liver tissue for pathological examination.
Results: There were no toxicological effects as evidenced by signs of mortality, behavior, diet consumption and tissue weights, however, some hematological parameters showed alterations in their value at higher dose level. The degree of protection was measured by using various biochemical parameters like total protein, albumin, BUN, AST, ALT and ALP levels. Nimbolide showed dose dependent hepatoprotective in nature which was further substantiated by marked decrease in incidence of hepatocellular necrosis on histopathological and transmission electron microscopic analysis.
Conclusion: This study suggests nimbolide possess hepatoprotective effect against CCl4 induced liver damage in rats with efficiency similar to that of Silymarin standard.
Nagappa S. Baligar*, Ravindranath H. Aladakatti, Mukhtar Ahmed and Murigendra B. Hiremath
KLES Kidney Foundation, KLES Prabhakar Kore Hospital and MRC 1, Belgaum-590010, Karnataka, India
19 February, 2014
31 May, 2014
28 June, 2014
01 August, 2014