EVALUATION OF ANTI- ANGIOGENESIS ACTIVITY OF NEEM ROOT USING ZEBRA FISH MODELAbstract
Introduction: Angiogenesis is the formation of new blood vessels which is a process involved in development of tumors and other neovascular diseases. Developmental angiogenesis in zebra fish embryos is a relevant and emergent model to evaluate the anti-angiogenesis activity which was used in present study. With this model, blood vessel formation and the blood flow can be clearly observed. Various parts of Neem tree were proved to have potential anticancer activity. Based on this fact, neem root has been selected for this study. Method: Zebra fish embryos were treated with different concentrations of Water soluble fractions of crude methanolic extract of neem root, Imatinib (standard) and control. Then, they were observed for 3 days within 24 hour interval. Results: Various phenotypical abnormalities that were observed during the development of larvae had been captured using digital microscope. Toxicity and survival rates were noted. Conclusion: Based on the results obtained, the water soluble fractions of methanolic extract of neem root was found to have the ability to inhibit in -vivo angiogenesis. Therefore, it can be concluded that, the study drug have the tumor growth inhibition properties.
L. P. B. Lavanya Uppuluri*, V. N. Garge, and V. J. Kadam
Department of Pharmacology, Bharati Vidyapeeth College of pharmacy, University of Mumbai, Navi Mumbai, Maharashtra, India
27 October, 2014
20 January, 2015
14 May, 2015
01 June, 2015