EVALUATION OF ANTI-ARTHRITIC, ANTI-INFLAMMATORY, AND ANTIOXIDANT ACTIVITY OF POLYHERBAL FORMULATION IN SPRAGUE DAWLEY RATS

Objective: This work aims to evaluate in in-vivo the Antiarthritic and Anti-inflammatory activities and in-vitro antioxidant activity on Poly Herbal Formulation in rats. Method: Arthritis is a disorder of change in joint architecture and ligament degeneration. In this polyherbal formulation, medicinal plants were Zingiber officinale (33.931mg), Piper nigrum (33.931), Piper longum (33.931mg), Spergularia daemia (30 mg), Delonix elates (30 mg), Acalypha indica (30 mg), and Carum copticum (101.79 mg), which are well-known plants throughout India. They are commonly used to treat various disorders like cancer, diabetes, arthritis, rheumatoid arthritis, atherosclerosis, and chronic inflammatory disorders. The quality of finished products was evaluated as per the World Health Organization’s guidelines for the quality control of herbal materials. Arthritis and inflammation were induced in both male and female Sprague-Dawley rats. Freund’s complete adjuvant (FCA) is used for antiarthritic effect. Carrageenan-induced is used for the anti-inflammatory effect of polyherbal formulation (Rheumatigo) was studied at doses of 250 and 500 mg/kg and the effects were compared with prednisolone (10 mg/kg) and diclofenac (20 mg/kg). Blood samples were collected for hematological analysis at the end of the study. The histopathological analysis was carried out before terminating the study. DPPH and H2O2 methods are used in polyherbal formulations having antioxidant properties and IC50 is known. Results: Polyherbal formulation showed significant antiarthritic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with Prednisolone’s. The antiarthritic activity of polyherbal formulation is supported by hematological analysis. Conclusion: The polyherbal formulation showed significant antiarthritic activity in both male and female rats.