EVALUATION OF ANTI – CANCER ACTIVITY OF BARK OF CRATAEVA NURVALA BUCH. HAM AGAINST THREE CELL LINESAbstract
The present study was aimed to evaluate in-vitro anti-cancer activity of ethanolic extract of Crataeva nurvala Buch. Ham. The method used for determining the anticancer activity was the MTT bio-assay using A549(Human Lung Carcinoma) cell line, Hela (Human Cervix)cell line and MDA-MB(Human Adenocarcinoma, Mammary Gland)cell line. The ethanolic extract of Crataeva nurvala bark at different concentration (10µg, 20 µg and 30 µg) was used as test for this bioassay. Vincristine30µg/ml (anticancer drug) was used as reference standard and blank as control. Optical density and % cell lysis was estimated at the end of the study. Extract showed promising anticancer effect. % Cell lysis was found to be in the range nearer to the reference standard for all the cell lines and optical density at 492nm is (0.575to 1.191) almost equal to that of reference standard i.e. 1.151 and always more than blank. The IC50 values were found to be less than 10μg against A549 cell line, the activities against HELA cell line were found to be 13μg and IC50 values were found to be 20μg against MDA-MB cell lines. These finding support that Crataeva nurvala bark extract was found to have a good anti-cancer activity.
N.S. Wagh* and N. J. Gaikwad
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Amravati road, Nagpur- 440033, Maharashtra, India.
21 April, 2014
17 June, 2014
31 July, 2014
01 November, 2014