EVALUATION OF ANTI-DIABETIC AND HEPATOPROTECTIVE ACTIVITIES OF AQUEOUS AND ETHANOLIC EXTRACTS OF ANISOMELES MALABARICA ROOTSAbstract
Anisomeles species are traditionally used for treating aliments like hysteria, amentia, dyspepsia, epilepsy, anorexia, diarrhoea etc. the present work, we aimed to evaluate the anti-diabetic activity and hepatoprotective activities of aqueous and ethanolic extracts of Anisomeles malabarica roots. Anti-diabetic activity was studied at three dose levels 100, 200, 400 mg/kg of aqueous and ethanolic extracts in normoglycemic and Streptozotocin (STZ) induced diabetic rat models. Tolbutamide (40 mg/kg) was used as standard treatment. Blood glucose levels were monitored at 1, 2, 4, 8, 16 and 24 h post-administration using glucometer. The hepatoprotective activity of the extracts was assessed at dose levels 100, 200, 400 mg/kg of aqueous and ethanolic extracts in ethanol intoxicated groups. Silymarin (100 mg/kg) was used as Levels of ALT, AST, ALP, and Bilirubin were measured using standard kits. Liver volumes and liver weights of the animals were also recorded. The results revealed that both aqueous and ethanolic extracts have anti-diabetic and hepatoprotective activities; however, the activity was more prominent with 400 mg/kg of ethanolic extracts and is comparable with Tolbutamide (40 mg/kg) and Silymarin (100 mg/kg) treatment groups, respectively.