EVALUATION OF ANTI-DIABETIC POTENTIAL OF CYPERUS PANGOREI ROTTB. ETHYLACETATE SUBFRACTIONS

A study was undertaken to fractionate and characterize the antidiabetic potential of Cyperus pangorei rhizomes. The column chromatography and HPTLC study was performed on ethyl acetate fraction and five sub-fractions were separated and subjected for the evaluation of antidiabetic potential in streptozotocin induced diabetic rats.  The experimental data indicated that the ethyl acetate sub-fractions SF₄ (26.39%) (P < 0.05) and SF₅ (43.69%) (P < 0.05) at the dose of 50mg/kg BW produced the maximum fall in the blood glucose level of diabetic rats after 15 days of treatment. Elevated levels of triglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL), very low density lipoprotein (VLDL) and decrease level of high density lipoprotein (HDL) and serum insulin level were observed in Streptozotocin-induced diabetic rats. While significant differences were observed in serum insulin level and serum lipid profiles in sub fractions treated diabetic animals, when compared with the diabetic control and normal animals (P < 0.01). In sub fractions SF₄ and SF₅ treated group decreased level of serum TG, TC, LDL and VLDL and increased serum insulin and HDL level has been observed. Histopathological observations revealed that SF4 and SF5 are non-toxic and restored the normal histoarchitecture of pancreatic cells. SF₄ and SF₅ offer a promising therapeutic value in prevention of diabetes. Further studies will be needed in future to isolate the active principles as well as identify the possible mechanism of active principle with its structural characterization.