EVALUATION OF ANTIGENOTOXIC POTENTIAL OF BEET ROOT EXTRACT AGAINST HAIR DYE INDUCED GENOTOXICITYAbstract
In-vitro investigations toward DNA damage reported that the biological effect of genotoxic exposures could influence the life cycles of different human cells. Sister chromatid exchange (SCE) assay is broadly used, sensitive cytogenetic biomarker of exposure to genotoxic agents. Naturally available products may play a significant role against the cytogenetic damage but available research data is not consistent. The purpose of this study was to evaluate the anti-genotoxic potential of beet root extract (BRE) against hair dye induced genotoxicity in cultured human peripheral blood lymphocytes (PBLs) using SCE assay. For this, PBLs were treated with different doses of hair dye ingredients along with BRE. Significant (p<0.05) rise in SCE frequency indicates genotoxicity of hair dye ingredients (p-Phenylenediamine-200 µg/ml, resorcinol-200 µg/ml, and hydrogen peroxide-6.168 µg/ml). Treatment of BRE along with hair dye showed significant (p<0.05) reduction in SCE frequency in a dose-dependent manner as compared to hair dye treated samples.
S. Kumar, V. Vishwakarma, B. Yadav, R. Gupta, N. K. Aggarwal and A. Yadav *
Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
31 January 2020
18 March 2020
20 March 2020
01 April 2020