EVALUATION OF ANTIHYPERGLYCEMIC ACTIVITY OF THE POLYHERBAL AND ALLOPOLYHERBAL FORMULATION ON NICOTINAMIDE-STREPTOZOTOCIN-INDUCED TYPE-2 DIABETIC RATSAbstract
Amla, Arjuna, Gudmar, Guduchi, and Ginger have been usually used in the traditional system of medicine for the treatment of diabetes. The aim of the present study to evaluate antihyperglycemic activity of polyherbal and allopolyherbal formulation on Streptozotocin-Nicotinamide (STZ-NA) induced type-2 diabetes rats. All the extracts of herbs used for the present study were obtained by supercritical fluid extraction. The Oral glucose tolerance test (OGTT) and anti-hyperglycemic activities were performed on STZ-NA-induced diabetic rats. The effects of metformin, PHF, and allopolyherbal formulation (APHF) on plasma insulin, hemoglobin, liver glycogen, and glycated hemoglobin (HbA1c) were analyzed. Statistical analysis was done by using one-way ANOVA followed by Dunnett’s multiple comparison tests. The OGTT of metformin, PHF, and APHF showed increased glucose tolerance. Single-dose anti-hyperglycemic activity of metformin, PHF, and APHF showed a significant (P<0.01) decrease in blood glucose level (BGL) in diabetic rats at 1st, 3rd, 5th, and 7th h. Multiple doses anti-hyperglycemic activity of metformin, PHF, and APHF showed significantly (P<0.01) reduction of BGL in diabetic rats after 14th, 21st, and 28th days of treatment. After treatment with metformin, PHF, and APHF for 28 days, there was a significant (P<0.01) decrease in HbA1c and a significant (P<0.01) increase in plasma insulin, hemoglobin, and liver glycogen levels in treated diabetic rats. Histopathology of the pancreas in treated groups showed the protection of β-cells of the pancreas. It is concluded that the simultaneous administration of polyherbal formulation and allopathic drugs increased the therapeutic effect, reduced dose and reduced side effects.
S. N. Belhekar *, P. D. Chaudhari and R. L. Jadhav
Department of Pharmacology, Gourishankar Institute of Pharmaceutical Education & Research, Limb, Satara, Maharashtra, India.
06 February 2020
12 October 2020
17 October 2020
01 November 2020