EVALUATION OF ANTIMICROBIAL ACTIVITY OF ASAFOETIDAAbstract
This study was carried out with the objective of the antibacterial and antifungal activity of chloroform, ethyl acetate, ethanol, methanol and aqueous extracts of Asafoetida. Antibacterial activity was carried out against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and the antifungal activity was evaluated against Aspergillus niger, Candida albicans. The testing was done by well diffusion method and evaluation was done by detecting zone of inhibition (in mm) and minimum inhibitory concentration (MIC). Studies were perfomed with two different concentrations of all extracts (2 mg/ml and 4 mg/ml). Zone of inhibition were compared with standards like Ciprofloxacin (0.1 mg/ml) and Fluconazole (0.1 mg/ml). The results showed that ethyl acetate, ethanol, and methanol extract has significant antimicrobial activity and highest activity was reported with methanolic extract. The minimum inhibitory concentration of methanolic extract, ethanolic extract and ethyl acetate extract against most of the test microorganisms were 1 mg/ml, 1 mg/ml, 2 mg/ml respectively.
S. D. Patil*, S. Shinde, P. Kandpile and A. S. Jain
Shri. D. D. Vispute College of Pharmacy and Research center, New Panvel, Maharashtra, India
27 June, 2014
28 August, 2014
29 October, 2014
01 February, 2015