EVALUATION OF BINDING EFFECT OF PROSOPIS CINERARIA LEAF AND STEM BARK EXTRACTS WITH CALF THYMUS DNAAbstract
The present research aimed to evaluate the binding effect of hydroalcoholic extracts of leaf and Stem bark of Prosopis cineraria (PCL and PCB) with Calf Thymus (CT) DNA. The interaction of the PCL and PCB with Calf Thymus (CT) DNA has been studied using absorption spectra, viscosity measurements and cyclic voltammetry methods. It was observed that the PCL and PCB exhibited hypochromic and bathochromic shifts in the absorption spectra on binding to DNA which is a typical characteristic of DNA intercalation. In viscosity measurements, varying concentration of PCL and PCB causes a significant increase in viscosity of DNA. The variations of the cyclic voltammetric behavior of both extracts PCL and PCB was noted which demonstrate intercalative interaction between the PCL, PCB and the CT-DNA. In conclusion, both PCL and PCB possessed good DNA-binding abilities.
Stellaa Robertson* and N. Narayanan
Department of Pharmacognosy, Jaya College of Paramedical Sciences, College of Pharmacy, Thiruninravur - 602024, Tamil Nadu, India
15 March, 2016
19 July, 2016
25 July, 2016
01 August 2016