EVALUATION OF EFFECT OF AYURVEDIC PREPARATIONS ON NEPHROPATHY IN DIABETIC WISTAR RATSAbstract
Introduction: Type 2 diabetes, which is the most common type, is often a result of excess body weight and physical inactivity in genetically predisposed individuals. Over due course of time diabetes can cause blindness, kidney damage, neurological problems, and coronary artery disease. Improvement in glycemic control is important factors in delaying the onset and progression of diabetes-related complications. In spite of the availability of newer antidiabetic drugs, prevention of complication is still a challenge. Nishamalaki, Patoladi Yoga and Shobhanjan are the Ayurvedic formulations claimed to be effective in preventing nephrotoxicity. Objective: To evaluate the therapeutic efficacy of Nishamalaki, Patoladi Yoga, and Shobhanjan in diabetic nephropathy. Materials and Methods: Total of 36 Wistar rats were used for the study. Diabetes was induced in 30 Wistar rats of either sex with STZ (35 mg/kg) i.p followed by High Fat High Fructose diet (HFHF), (coconut oil+ vanaspati) till the development of Diabetic nephropathy (10wks) which was confirmed by Sr. creatinine, blood urea levels. Out of six groups included in the study, group I was without diabetes, Diabetic animals were divided into five groups Group II-Diabetic control, Group III- Nishamalaki, Group IV- Patoladi Yoga, Group V- Shobhanjan & Group VI- Enalapril and drug treatment was given according to groups orally for 45days. Blood sugar levels, BUN, creatinine were tested weekly. Results: Nishamalaki significantly (p<0.001) improved Creatinine level compared with the diabetic control group. Reduction in BUN values was more with Patoladi (p<0.001) than Shobhanjan (p<0.01). Results of Nishamalaki, Patoladi yoga, and Enalapril were comparable to the control group. Patoladi appeared significantly more effective in reducing BUN & Creatinine values than shobhanjan. Nishamalaki additionally showed a blood sugar lowering effect. Conclusion: Nishamalaki, Patoladi yoga has shown a protective effect against diabetic nephropathy. Both are effective in delaying the progression of nephropathy in diabetes mellitus.
M. S. Bhosale, J. S. Dawane, V. A. Pandit * and P. S. Khatavkar
Department of Pharmacology, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, Maharashtra, India.
14 November 2018
30 March 2019
13 July 2019
01 August 2019