EVALUATION OF HYDROCHLOROTHIAZIDE AND METHYLDOPA SIMULTANEOUSLY BY APPLYING CHEMOMETRIC SPECTROPHOTOMETRIC METHOD

In the present work, simultaneous estimation of Hydrochlorothiazide and Methyldopa was carried out by applying chemometric assisted UV spectro-photometric methods. The Two chemometric methods, classical least square (CLS) and inverse least square (ILS) were applied for estimation of Hydrochlorothiazide and Methyldopa simultaneously in tablets without any prior separation, and no graphical treatment of the overlapping spectra of these drugs were required. The chemometric calculations for CLS and ILS methods were performed by using the Chemometrics Toolbox 3.02 software equipped with MATLAB 6 and excel. The results derived from these two chemometric methods were compared statistically. These two chemometric methods were successfully applied estimation of two drugs from their marketed tablet dosage forms without any prior separation procedure. The methods were validated according to ICH guidelines. The results of the recovery study were found to be closer to 100 percent, and relative standard deviation obtained for different parameters of ILS and CLS methods was found to be within a limit for both the drugs. The two chemometric methods mentioned in this study can be satisfactorily applied for the quantitative analysis of Hydrochlorothiazide and Methyldopa from their dosage form.