EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF FLOWERS OF BLEPHARIS MOLLUGINIFOLIAAbstract
The objective of this study was to characterize the non-enzymatic and enzymatic antioxidants and Scavenging activities of different extracts (Petroleum ether, Benzene, Chloroform, Acetone, Ethanol, Methanol and Water) of dried flowers powder of Blepharis molluginifolia (Acanthaceae). Enzymatic antioxidant activity such as Superoxide Dismutase, Glutathione –S-transferase, peroxidase and Catalase of B. molluginifolia flowers were high in methanol extract. Non enzymatic antioxidants like Tocopherol, Ascorbic acid, Phenols, Carotenoids and Lycopene were analysed. Acetone extract showed highest Total Phenol content and Vitamin-C were 100.66±1.5 mg GAE/gm & 52.08±2.42 mg/gm respectively. Scavenging activities like DPPH, ABTS and H2O2 were high in Methanol extract. In this study, the non-enzymatic antioxidant activity was found to be significant, which prove to be a better scavenger of free radical in comparison to enzymatic extracts in the shade dried extracts.
S. Deepika* and S.V. Rajagopal
Research Scholar, Department of Biotechnology, GITAM Institute of science, GITAM University, Visakhapatnam, Andhra Pradesh, India
15 December, 2013
10 March, 2014
25 April, 2014
01, June 2014