EVALUATION OF IN VITRO IMMUNOMODULATORY EFFECTS OF SOYABEAN (GLYCINE MAX. L) EXTRACTS ON MOUSE IMMUNE SYSTEMAbstract
To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabean, the present study was intended to evaluate the in-vitro immunomodulatory activity of dry seeds of Glycine max. L. Effect of ethanolic and methanolic extracts of soyabean was evaluated at various concentrations (800μg/ml to 6.25μg/ml) for secretion of various mediators. Immunomodulatory activity of both extracts was examined on murine macrophage phagocytosis by Nitroblue tetrazolium (NBT) dye reduction, nitric oxide, lysosomal enzyme activity and myeloperoxidase activity. Proliferation of spleenocytes and bone marrow cells was evaluated by 3 (4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) as mitogen. Both aqueous and acetone extracts presented immunomodulatory activity in peritoneal mouse macrophages, bone marrow cells and spleenocytes without clear dose response relationship. In-vitro phagocytic index and proliferation index showed significant results and thus proving the need for confirmation through in-vivo studies.
T. Anitha, K. Parameswari, B. Kishori and R. Usha *
Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati – 517502, Andhra Pradesh, India
25 September, 2014
16 December, 2014
19 January, 2015
01 May, 2015