EVALUATION OF NOOTROPIC ACTIVITY OF HYDROXYCITRIC ACID IN PHENYTOIN TREATED RATSAbstract
The present study investigates neuroprotective effect of hydroxy citric acid (50%) on phenytoin induced cognitive impairment and oxidative stress in rats.Male Wistar rats were divided into 6 groups and each group received vehicle, phenytoin (25mg/kg, i.p) and hydroxy citric acid (400 and 800mg/kg, orally) for 14 days respectively. Memory and learning was evaluated by using the Rectangular maze, Morris water maze and Locomotor activity were evaluated. On day 14, animals were sacrificed, brain tissue was dissected. The Acetylcholine Esterase (AChE) levels, antioxidants levels were estimated in the brain homogenate.The histopathological studies were done to check for the gross changes in histology. Phenytoin (25mg/kg, i.p) produced significant deficits in learning and memory as indicated by significant increase transfer latency in rectangular maze, Morris water maze and decrease number of counts in Actophotometer. It also produced significant elevation in brain AChE, MDA levels along with reduction in Catalase and DPPH levels. Hyrdroxy citric acid (400 and 800mg/kg) orally, when co-administered with phenytoin, significantly prevented phenytoin induced cognitive impairment, oxidative stress and improved the histopathological abnormalities in a dose dependant manner.
Mada Mamatha, Thumuganti Priyanka, Swathi Konda and Meesa Madhavi*
Assistant Professor, Department of Pharmacology, Vaagdevi College of Pharmacy, Ramnagar, Hanamkonda, Warangal – 506001, Andhra Pradesh, India
15 December, 2013
05 February, 2014
25 April, 2014
01, June 2014