EVALUATION OF PESTICIDE CHLORPYRIFOS TOXICITY ON DROSOPHILA MELANOGASTERAbstract
Chlorpyrifos is one of the commonly detected pesticides found in food and water. Extensive use of it in agriculture and its persistence in the environment have raised public concern to overcome the pollution and toxicity problems. Exposure to chlorpyrifos poses several harmful effects to animal and human health. And the pathology in neurodegenerative diseases is mainly because of mitochondrial dysfunction. GSH depletion proceeding with mitochondrial dysfunction is therefore considered as the earliest triggering factor of neurodegeneration. In the present study using Drosophila melanogaster a fly model for human neurodegenerative disease, we investigated the effects of chlorpyrifos on glutathione, reactive oxygen species, mitochondrial Complex I, and Complex II activities. We found that chlorpyrifos generates reactive oxygen species, diminished the levels of reduced glutathione (GSH) concurrently increasing oxidized form of glutathione (GSSG), this preceded with inhibition of mitochondrial enzymes such as complex I and complex II activities, ultimately leads to motor dysfunction and death of Drosophila flies.
H. N. N. Kumar, M. Raveesh, A. K. Shettar and P. Niranjana *
Post-graduate Studies and Research in Biochemistry, Kuvempu University, Shankaraghatta, Karnataka, India.
14 April 2020
17 July 2020
22 October 2020
01 April 2021