EVALUATION OF PHYTOCHEMICAL AND PHARMACOLOGICAL ASPECTS OF EPIPHYTIC ORCHID LUISIA ZEYLANICA LINDL.Abstract
The present study was carried out to evaluate phytochemical composition and pharmacological activities of leaf extract of Lusia zeylanica by using different solvents. The solvents like n-hexane, ethyl acetate and methanolic were used to extract dried leaf material of L. zeylanica. These extracts were analyzed for phytochemical constituents with GC-MS analysis and antimicrobial activity tested against four bacterial strains and three fungal strains by using the agar diffusion method. In-vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenols, saponins, tannins, and terpenoids. GC-MS analysis determines the presence of 11 compounds in ethyl acetate, 7 compounds in methanolic extracts and a total of 7 unknown compounds respectively. A significant cancer cell growth inhibition was observed for two extracts with IC50 values ranging between 18.36 µg/ml to 67.914 µg/ml. Our result shows this plant is a promising source of phytocompounds with potential antimicrobial and anticancer activity.
J. Katta, V. Rampilla * and K. S. Mohamad
Dr. L. H. R. Government Degree College, Mylavaram, Krishna, Andhra Pradesh, India.
22 May 2019
18 January 2020
05 February 2020
01 March 2020