EVALUATION OF PHYTOCHEMICALS AND IN VITRO PHARMACOLOGICAL ACTIVITY OF ACACIA SINUATA PLANT LEAVESAbstract
Objective: The present work is aimed to screen phytochemicals, in vitro antimicrobial and antioxidant activity of the non polar and polar extracts of Acacia sinuata. Methods: Leaves of Acacia sinuata– were collected, dried and powdered. The powdered material is subjected to soxhlet extraction using various polar to non polar solvents and allowed for evaporation. The crude extracts thus obtained were used for further screening of phytochemicals, in vitro antimicrobial activity and antioxidant activities. Results: Phytochemical evaluation of Acacia sinuata proved that most of the secondary metabolites are extracted in polar solvents than non polar solvent like hexane. All the extracts of the Acacia sinuata possess significant amount of phenols and flavonoids and they also showed antimicrobial and antioxidant activity. Out of all the extracts of Acacia sinuata, methanol extracts showed better antibacterial, antifungal activity and antioxidant activity. Conclusion: The present work concluded that the methanol extract of Acacia sinuata was useful to develop new plant based dugs against various pathogenic bacteria as well as fungal species.
V. E. Vijayasekhar, M. Satya Prasad, D. S. D. Suman Joshi, K. Narendra, A. Krishna Satya and K. R. S. Sambasiva Rao
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, India
14 January, 2016
12 March, 2016
17 March, 2016
01 June 2016