EVALUATION OF THE ANTI-DIABETIC ACTIVITY OF THE TRAN VIDHAI KUDINEER ON FRUCTOSE AND STREPTOZOTOCIN INDUCED DIABETIC RATS

Aim and Objectives: The present study was an attempt to investigate the effect of Thetran vidhai kudineer (TVK) on streptozotocin (STZ) induced diabetes in male Wistar rats. Methods: TVK, is a Siddha polyherbal formulation containing four ingredients includes Strynchnos potatorum, Terminalia chebula, Cassia auriculata, and Limonia acidissima. 6-8 week old 30 male Wistar albino rats fed with normal diet ad libitum and free access to water containing 10% fructose (w/v) for 5 weeks. After five weeks of dietary manipulation, all groups except the normal control group received a single injection intraperitoneal (ip) of STZ (40 mg/kg b.w.) dissolved in citrate buffer (pH 4.4). One week after the STZ injection, animals with non-fasting blood glucose levels > 250 mg/dl were considered as diabetic. Four groups of STZ-induced diabetic rats were orally treated with metformin (100 mg/kg) and TVK (300, 600, and 1200 mg/kg b.w), respectively. Bodyweight recording was done weekly; fasting blood glucose levels were measured at the end of 8^th week, 10^th week, 12^th week. Lipid profile, SGOT, SGPT, Serum Insulin, Serum creatinine, TNF-alpha were measured at the end of the 12^th week. Results: TVK and metformin were found to be reducing the blood glucose level, lipid profile, creatinine level, SGOT, and SGPT levels when compared to diabetic control, whereas the treatment with TVK increased body weight, HDL levels, TNF-alpha levels, and insulin levels when compared to the diabetic control. Histopathological studies reinforce the healing of damaged organs by TVK and metformin as a possible mechanism of their anti-diabetic activity. Conclusion: Thus, it may be concluded that TVK, in addition to the anti-diabetic activity, also possess anti-hyperlipidemic activity in the fructose and STZ induced diabetic model.