EVALUATION OF THE ANTI – PROLIFERATIVE EFFECT, ANTIOXIDANT AND PHYTOCHEMICAL CONSTITUENTS OF FICUS PUMILA LINN.Abstract
This study sought to evaluate the heavy metal content of the raw powder and extract of the plant, the phytochemical constituents, antioxidant effect by the use of DPPH assay, the total phenolic content using Folin Ciocalteu assay and the cytotoxic effect using the MTT Assay of Ficus pumila ethanolic extract, methanolic and hydro fractions on liver cancer cells (HepG2), Leukemic cells (Jurkat) and normal liver cells (Chang). FTIR and Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify the functional groups and major constituents of the most active fraction of F. pumila. Alkaloids, terpenoids, flavonoids, cardiac glycoside, saponins, and tannins were present in the ethanolic extract of F. pumila. The heavy metal analysis revealed the presence of Iron in both the raw powder (1.97 ± 0.11 mg/l) and extract (0.92 ± 0.02 mg/L). Zinc was also detected in both the raw powder (1.19 ± 0.00 mg/l) and extract (0.6595 ± 0.02 mg/l). The results from the FTIR revealed the presence of alkynes, alkyl halides, aromatics and aliphatic amines common to all fractions and compounds such as Phenol, 2,4-bis (1,1-Dimethylethyl) and Dodecane, 2,6, 10-trimethyl were detected in the samples by GC-MS. The DPPH assay also showed that all the fractions scavenged DPPH free radical in a dose-dependent manner as compared to the positive control (Ascorbic acid) and positively correlated to the phenolic contents. The MTT assay revealed that methanolic fraction was selective towards the Jurkat cell lines (Selectivity Index = 2.822). This increases the prospects that this plant contains compound(s) which could serve as leads for novel anticancer drugs.
D. Torkornoo *, C. Larbie, S. Agbenyegah, J. N. N. Dowuona, R. Appiah-Oppong, E. Dotse, A. Aning, B. Adu and C. Sinclear
Center for Molecular Biomedicine, Institute for Molecular Cell Biology, Jena University Hospital, Jena, Germany.
24 August 2018
29 November 2018
05 April 2019
01 May 2019