EXPLORING ANTIOXIDANT ACTIVITY AND DNA PROTECTION POTENTIAL OF CHONEMORPHA FRAGRANSAbstract
Chonemorpha fragrans (Moon) Alston is a liana belongs to family Apocynaceae. Due to its ethanobotanical importance, it is used intraditional medicinal systems as a part of various medicinal preparations. In the work presented here the plant has been explored for its antioxidant potential and DNA protection ability. The antioxidant potential of C. fragrans was determined using DPPH, FRAP, Phosphomolybdanu and H2O2 Scavenging Capacity. Phenol and flavonoid content was measured followed byCorrelation analysis between antioxidant activities andphenols and flavonoid content. In addition, DNA protection ability of extracts was evaluated using double stranded plasmid DNA. C. fragrans extracts have shown significant antioxidant potential. Phenol content showed high correlation with Phosphomolybdenum reduction potential where as flavonoid content with Phosphomolybdenum as well as H2O2 radical scavenging potential. All the extracts exhibited an ability to protect the DNA against devastating effects of Fentons reagent except methanol roots, stem bark, in vitro and callus extracts. The extracts have shown significant antioxidant activityand Phenol, flavonoid content was found to contribute towards antioxidant potential of C. fragrans. The extracts also showed the DNA protection potential pointing toward its pharmaceutical importance.
Pradnya Kedari and Nutan Malpathak *
Department of Botany, University of Pune, Ganeshkhind Road, Pune 411007, Maharashtra, India.
27 April, 2014
27 June, 2014
08 August, 2014
01 December 2014