EXPRESSION OF CHICKEN ANEMIA VIRUS VP3 GENE IN HeLa CELLsAbstract
Chicken anemia virus (CAV) encodes the protein VP3 also named “apoptin” that specifically induces apoptosis in human tumor cells. In this study, the VP3 gene cloned in p VAX1 plasmid vector was transfected in HeLa cells. The expression of CAV VP3 gene was detected using immunoperoxidase test and indirect fluorescent antibody technique. High level of gene expression was observed in HeLa cell culture. Successful expression of recombinant plasmid in mammalian cell lines shows that the plasmid could be further evaluated in field trials and hence useful tool for study of antitumor activity.