EXPRESSION OF GLUCOSAMINE-6-PHOSPHATE ISOMERASE AND JACOB GLYCOPROTEIN DURING CULTURE OF ISOLATES OF ENTAMOEBA HISTOLYTICAAbstract
The glucosamine-6-phosphate isomerase and Jacob glycoprotein gene expression levels were assessed during E. histolytica excystation from the feces of five patients. Excystation was performed at 0, 24, 48 and 96 h in Robinson medium, and RNA was extracted from the cysts and trophozoites. cDNA was synthesized to amplify the genes using real-time RT-PCR. The results demonstrated differences according to the incubation time and/or conversion of the cystic phase to the trophozoite, where the most important expression in the genes was observed at 96 h excystation. The expression levels of the genes studied positively correlated with the population of trophozoites during excystament
E. Jimenez-Cardoso,* MP. Crisostomo-Vazquez, L. Eligio- Garcia, VA. Marevelez- Acosta, P. Antonio- Cruz and A. Flores-Luna
Hospital Infantil de Mexico Federico Gomez, Laboratory of Parasitology Research, Dr. Márquez #162 Col. Doctores, 06720 México, D.F.
17 June, 2014
19 December, 2014
19 January, 2015
01 February, 2015