EXTRACTION AND CHARACTERIZATION OF GYMNEMAGENIN IN GYMNEMA SYLVESTRE LEAVESAbstract
The objective of the present investigation is to extract and characterize the active constituent, Gymnemagenin in Gymnema sylvestre leaves using various Chemical and Analytical techniques. Extraction of Gymnema sylvestre leaves powder is done by Continuous soxhlet extraction with 80% ethanol. Identification was done by various chemical tests and TLC. Characterization and quantification by HPLC method, which is quick, reliable and sensitive. LC system Shimadzu with isocratic pumps LC 20-AT, and U.V Detector SPD 20-A was used. Reverse Phase Column C18 (250mm x 4.6mm, 5A° particle size) and a mobile phase consisting of 100 mM ammonium acetate buffer: acetonitrile (75: 25, v/v) were employed. Chemical tests and TLC revealed the presence of Gymnemic acid and its aglycone Gymnemagenin. TLC profile revealed Rf value of 0.41 for Gymnemagenin in the extract and a retention time of 7.366 minutes in HPLC method with a concentration of 3.62 µg/ml in the extract. Various validation Parameters were developed from the HPLC method for identification and quantification of gymnemagenin.