EXTRACTION AND SECONDARY METABOLITE ANALYSIS OF COSCINIUM FENESTRATUM (GAERTN.) COLEBR: AN IMPORTANT MEDICINAL PLANT OF WESTERN GHATSAbstract
The aim of this study was to extract the secondary metabolites of Coscinium fenestratum stem extracts and to determine the alkaloid berberine using HPLC. Phytochemical analysis was carried for acetone, chloroform, methanol and Aqueous fractions of stem extracts. Total phenolic content and total flavonoid contents were estimated using standard Gallic acid and Quercitin respectively. TLC and HPLC were performed for alkaloid to determine the berberine content present in the stem extract. Preliminary phytochemical screening revealed the presence of alkaloids, saponins, steroids, phenolic substances and cardiac glycosides. Steroids and tannins were absent in the stem extract. The stem methanolic extract of C.fenestratum contained large amount of phenolics18.35±0.56mg GAE/g extract and the flavonoid was found to be 12.8±0.88 mg QE/g extract respectively. The concentration of berberine in the stem was found to be 0.2462%.
Santhosh Wilson Goveas* and Asha Abraham
Father George Albuquerque Pai Cell and Molecular Biology laboratory, Post Graduate Department of Biotechnology, St. Aloysius College (Autonomous), Mangalore-575 003, Karnataka, India
22 February, 2014
09 April, 2014
20 June, 2014
01 August, 2014