FABRICATION OF CHITOSAN ALGINATE NANOPARTICLE BASED FIBRE OPTIC BIOSENSORAbstract
In this work, a highly sensitive and selective nanoparticle-based fluorometric biosensor has been reported for the detection of asparagine levels. The L-asparaginase loaded chitosan alginate nanoparticles of size 396 nm with 600 µL of L-asparaginase (44.4 IU/mg) showing 83.5% entrapment efficiency was fabricated via ionic gelation method. In the presence of enzyme L-asparaginase (biocomponent) the substrate L-asparagine gets hydrolyze into L-aspartate and ammonium ions. The ammonium ions released were detected by a change in fluorescence intensity of pH-sensitive dye (Rhodamine 6G) immobilized along with L-asparaginase loaded chitosan alginate nanoparticles on the fiber optic probe. The developed fiber-optic biosensor under optimal conditions showed sensitivity in a detection range of 10-10 to 10-1M concentration with a detection limit as low as 0.04 × 10-10 M within a response time of 2 min. The fabricated biosensor demonstrated good performance analysis in terms of reversibility, reproducibility, selectivity, and stability. The developed biosensor has the potential to be used for the determination of L-asparagine in the diagnosis of leukemia, and in the food industry, it can be utilized for the determination of the quality of food.