FORMULATION AND EVALUATION OF GOAT FAT AND SHEA BUTTER BASED LIPOSPHERES OF BENZYL PENICILLIN
AbstractLipospheres of benzyl penicillin were formulated using the conventional thin film hydration technique. Five different combinations of shea butter, surfactant (Span 80) and goat fat were the key variables employed in the formulations. The resultant lipospheres were evaluated with respect to surface morphology, particle size distribution, encapsulation efficiency, in vitro drug release, in vivo bioavailability and in vitro antimicrobial activity. Particle size was found to increase with increased drug loading, the average particle radius of the batches being 12.5 nm. The encapsulation efficiency was found to be high at all levels with encouraging values of 80.21 and 83.44 % for batch A and E respectively, with batch A containing shea butter and span 80 in the ratio of 1:1 and batch E containing shea butter, span 80 and goat fat in the ratio of 1:2:1. Batch A appear to exhibit sustained release in vitro with cumulative drug release being 60 % while batch E has a cumulative drug release of 98 % respectively. The presence of goat fat however seems to impact negatively on the in vivo stability of batch E liposphere. The test micro-organisms showed sensitivity to the two batches in marked contrast to the uncapsulated benzyl penicillin especially against the multiple-antibiotic resistant strains of S. typhi, P. vulgaris and P. aereuginosa used in the study.
Article Information
11
1022-1027
578KB
1346
English
IJPSR
C.O. Esimone , A.A. Attama , U.E. Osonwa , C.D. Nwakile* and F.T.O. Onochie
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
18 December, 2011
21 February, 2012
29 March, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(4).1022-27
1-APR-2014