FORMULATION AND EVALUATION OF VERNONIA ELAEAGNIFOLIA PHYTO-FORMULATION FOR THE MANAGEMENT OF HYPERLIPIDAEMIAAbstract
The aim of the present investigation was to formulate and evaluate phytoformulation prepared from the ethanolic extract of Vernonia elaeagnifolia. Vernonia elaeagnifolia whole plant was collected and extracted with ethanol by soxhlation. Dried ethanolic extract of Vernonia elaeagnifolia was used to formulate the nanosuspension by homogenization method to enhance the bioavailability of phytoconstituents by increasing its solubility. Nano suspension of Vernonia elaeagnifolia (NS-VE, 50 mg/kg, 100 mg/kg) was evaluated for particle size, polydispersed index (PDI), entrapment efficiency, zeta potential, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC).NS-VE was screened for antihyperlipidemic activity with propylthiouracil induced and triton X-100 induced hyperlipidemia models in rats. The average particle size of NS-VE was found to be 0.027µm, PDI 60% (0.026 µm), 5mV zeta potential, and 46% entrapment efficiency. NS-VE (at doses 50 mg/kg, 100mg/kg bd.wt p.o) showed significant (p<0.01) anti-hyperlipidemic effect by reducing levels of TG, TC, LDL, VLDL and shown raised level in HDL when results were compared with normal control, disease control, and standard group. Nanosuspension of Vernonia elaeagnifolia can be used for the management of hyperlipidemia and other metabolic disorders with improved bioavailability.
S. Nawale *, K. P. Laxmi, M. S. Bhargavi and M. G. Raju
Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Osmania University, Bachupally, Hyderabad, Telangana, India.
10 November 2019
25 March 2020
28 March 2020
01 November 2020