FORMULATION, DEVELOPMENT AND IN-VITRO EVALUATION OF LOPINAVIR LOADED SOLID LIPID NANOPARTICLESAbstract
Lopinavir (LPV) is potent and poorly bioavailable molecule which is frequently administered protease inhibitors in the treatment of the HIV. Solid Lipid Nanoparticles (SLNs) of LPV were prepared using High Speed Homogenization. LPV were successfully encapsulated in the mixture of Glyceryl Behenate and Glyceryl monostearate. Poloxamer 188 was used as a surfactant for the preparation of the SLNs. Formulation composition and process parameter were optimized to get the desired quality of the SLNs. Formulation PF5 have mean particle size 185 ± 5 nm with Polydispersity index 0.11 ± 0.01 which indicates very narrow particle size distribution. % Entrapment efficiency was 86.2 ± 1.5. Slow drug release profile indicates the homogeneous dispersion of LPV in lipid matrix. SLNs have spherical shape and smooth surface which has been confirmed using SEM analysis. XRPD spectra show the reduction in crystalline behaviour of the drug and the lipid after formation of the SLNs. There was no significant change in the mean particle size and Polydispersity index after 6 month storage at 25°C/60% RH.
Kaushal P. Patel*, Chirayu J. Pathak and Rakesh P. Patel
Department of Pharmaceutics & Pharmaceutical Technology, S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva - 384012, Mehsana, Gujarat, India.
02 June 2014
12 August, 2014
16 November, 2014
01 January, 2015