FTMS-ESI CHARACTERISATION OF ETHER EXTRACT OF AZADIRACHTA INDICA LINN. OBTAINED FROM ANANTHAPURAM ANDHRA PRADESH INDIA

Neem (Azadirachta indica) is a member of the Meliaceae family and its role as the health-promoting effect is attributed because it is a rich source of potent biochemicals. It has been widely used in Chinese, Ayurvedic, and Unani medicines worldwide especially in Indian Subcontinent in the treatment and prevention of various diseases. In this present study’s author being investigated and identified biochemically active components in ether and methanolic extracts of stem bark of Azadirachta indica collected from the nursery of Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India. The stem of the plant washed with water and methanol; dried plant material was successively extracted with petroleum ether at 34 ºC by Soxhlet apparatus for 72 h. The chemical formulas, double band equivalence and percentage of occurrence of the compounds have been elucidated with Fourier Transform Mass spectrometry with ESI detector in the negative mode as well as positive mode. The Instrument was run in full mode m/z (50-1000). METLIN database was used to identify the compounds. Many compounds were identified with m/z A.128.0346, B.273.1506, C.377.0868, etc., among those we have identified two important peaks in the negative mode of analysis, one is at 215.0345 with double bond equivalent 9.5 and percentage of occurrence 2.9423 ppm. When we compare this peak data in METLIN database, we tentatively 6 compounds were identified. The second peak was observed at m/z 387.1234 with double bond equivalent 15.5 and percentage of occurrence 1.8867 ppm; this was also compared with METLIN data identified compound was calomelanol with molecular formula C₂₄H₂₀O₅.