FUNGICIDAL ACTIVITY OF FIVE DISINFECTANTS ON ISOLATES OF CANDIDA SPECIESAbstract
Determine the fungicidal activity of five disinfectants on Candida auris of clinical and environmental origin. Assess the fungicidal efficiency of each disinfectant on the microorganism. Calculate the percentage of efficiency (% E) of each disinfectants on Candida strain and determine the specific death rate (k) and decimal reduction time for microorganisms. Analytic and experimental research with quantitative methods. We realized a strain’s workbench by culturing it in corresponding culture media. Analysis was a challenge essay, performed on four surfaces and at different exposure times; 1, 5, 15, and 30 minutes of contact between disinfectant and yeast. Once the disinfection process was carried out, what was expected was that after 15 minutes of contact between the yeasts and the disinfectants, a percentage of inhibition of the microorganism between 90 and 95% was presented, thus guaranteeing the duration of elimination of the infectious agent by disinfectants. Results show that after one minute of contact between Candida auris and disinfectant in a gloved hand, the yeast was removed when the clinical and environmental strains were analyzed. Data showing disinfectants efficiency percentages greater than 90% overstrains of Candida used in the study. Decimal reduction times in seconds of the strains of Candida against the disinfectants used in the study were between 9.3 and 14.04 s. It was possible to evaluate effectiveness using the inhibition percentage of each disinfectant on the strains of Candida auris of clinical and environmental origin. Similarly, specific death rate and decimal reduction times in seconds were determined for strains under study.
Helena Lucía Martínez-Bajaire, Claudia Marcela Parra, Jorge Alberto Cortes and Janeth Arias Palacios *
Biotechnology Ambiental and Industrial Microbiology Group, Internal Medicine Department, Pontificia Universidad Javeriana, Faculty of Sciences, Department of Microbiology, Bogota, Colombia.
22 September 2021
29 September 2022
23 May 2022
01 June 2022