GC-MS ANALYSIS AND ANTI-INFLAMMATORY ACTIVITY OF MUREX TRIBULUSAbstract
The aim of this study was to screen the presence of bioactive compounds through GC-MS analysis and carry out the ant-inflammatory test of the methanolic tissue extract of Murex tribulus, a spiny predatory gastropod that is widespread from the Central Indian Ocean to Western Pacific Ocean. The GC-MS analysis of the methanolic flesh extract revealed the presence of four bioactive compounds, 1, 2 dimethyl hydrazine, urea, cis-9 hexadecenoic acid, and 1, 1, 1, 3, 5, 5, 5 hepta-methyltrisiloxane. Among the compounds identified, 1, 1, 1, 3, 5, 5, 5-Heptamethyltrisiloxane was the most abundant antimicrobial compound (92.87%) present in the methanolic tissue extract of M. tribulus. The results of the GC-MS analysis showed that the bioactive compounds present in the tissue extract showed anti-inflammatory responses. Hence in-vitro anti-inflammatory activity in the tissue extract of M. tribulus was determined by proteinase inhibitory activity and 5-lipooxygenase inhibitory assay. The anti-inflammatory test showed promising results with high percentage of inhibition such as 53.50% at a concentration of 1000 µg/ml in proteinase inhibitory activity and 75.42% at the concentration of 1000 µg/ml in %-lipoxygenase inhibitory assay.
J. E. Mereen * and J. H. J. Diaz
Department of Zoology, St. Mary’s College (Autonomous), Thoothukudi, Affiliated to Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India.
04 December 2019
10 October 2020
20 November 2020
01 December 2020