GC–MS ANALYSIS AND IN-VITRO CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF ANTIGONON LEPTOPUS FLOWERS
AbstractThe present study has been designed to evaluate the bioactive compounds and cytotoxic activity from the n-hexane fraction of methanolic extract of Antigonon leptopus flowers. The n-hexane fraction was analysed by GC-MS (Thermo GC-TRACE ultra ver.: 5.0, Thermo MS DSQ II) which revealed the presence of many diversified compounds including flavonoids, Vitamin D2 derivative, carbohydrates, amino acids, quinazolines etc. All the compounds were identified from Wiley spectral library. Mass spectral data of 16 compounds is presented. The in vitro cytotoxic activity of n-hexane fraction of the methanolic extract was performed by MTT assay method against CHOK 1cell line (Chinese Hamster Ovary cell line) and A-549 cell line (Human Lung adenocarcinoma epithelial cell line).Under the experimental conditions the cytotoxic property was not observed for n-hexane fraction. From the results it is evident that Antigonon leptopus flowers contain various medicinally active phytocomponents .The absence of cytotoxic activity against CHOK 1cell line and A-549 cell line recommends the safety of the Antigonon leptopus flowers
Article Information
55
3083-87
637
1719
English
Ijpsr
S. S. Prajna , K. Sony Priya, S. Swarnalatha, P. Bhojaraju, L. K. Kanthal and K. Satyavathi*
Avanthi institute of pharmaceutical sciences, Cherukupally, Bhogapuram, Vizianagaram , Andhra Pradesh, India
ksatyacharan@yahoo.com
26 November, 2014
23 January, 2015
27 March, 2015
10.13040/IJPSR.0975-8232.6(7).3083-87
01 July, 2015
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