GC-MS ANALYSIS AND IN-VITRO CYTOTOXICITY STUDY OF DENDROPHTHOE FALCATA AND TRIDAX PROCUMBENS EXTRACTS AGAINST HACAT CELLS LINEAbstract
The present study shows the cytotoxic effect of Dendrophthoe falcata (L. f) and Tridax procumbens Linn. extracts against HaCat (Skin cancer) cell line by MTT and SRB assay method. The phytochemical screening of extracts dichloromethane: methanol (DM), methanol: water (MW), and ethanol: water (EW) revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, sterols, and triterpenes. % Cell inhibition by MTT assay of DFDM extract shows 90.00 ± 0.09% and TPDM 91.29 ± 0.02% at 80 µg/ml. IC50 values of DFDM extract <20 µg/ml and TPDM extract 20 µg/ml compare to IC50 values of standard 5-fluorouracil <10 µg/ml by MTT assay. SRB assay method DFDM extracts shows 86.96 ± 0.07% and TPDM shows 89.14 ± 0.27 with IC50 values < 20 µg/ml compare standard 5-fluorouracil <10µg/ml. HPTLC fingerprint analysis of active DFDM extract showed 11 peaks at 366 nm Rf values 0.06-0.87 & TPDM shows showed 12 peaks Rf values 0.33- 0.83 at 366 nm. Active extracts were analyzed agilent 7890A GC coupled with triple quadrupole mass detector. GC–MS analysis DFDM extract shows presence of the 16 phytocompounds, major are [Diethetyl phosphonate- (RT-6.615, 166.075, 43.8.%), Benzyl oxy tridecanoic acid (RT-6.730, MW-320.235, %A-44%), 9(2-phenyl ethyl) Heptadecane (RT-10.233, 344.344,29.1 %), Hexadecanoic acid butyl ester (RT-15.099, 312.302, 65.5%), Linoleic acid, 2,3 bis (O-TMS) propyl esters (RT-23.098,26.3%)]. TPDM extracts shows 19 phytocompounds, major are [Tetradecene (RT-8.249, 198.23, 32.8%), Cyclohexasulfide (RT-9.893, 191.83, 71.4%), Cyclic octaatomic sulphur (RT-15.810, 66.7%), Squalene (RT-22.081, 24.1%). The study showed that the phytochemicals present in the leaves extracts of D. falcata and T. Procumbens were responsible for cell inhibitory activity against the HaCat cell line.
V. C. A. Bhagat * and M. S. Kondawara
Department of Quality Assurance, Appasaheb Birnale College of Pharmacy, Sangli, Maharashtra, India.
03 April 2020
27 July 2020
16 August 2020
01 April 2021