GC-MS ANALYSIS OF LEAF EXTRACT OF GYNOCHTHODES UMBELLATA (L.) RAZAFIM. AND B. BREMER (RUBIACEAE)Abstract
The present investigation was carried out to determine the presence of volatile, bioactive, phytocomponents in the leaf ethanolic extract of Gynochthodes umbellata (Syn. Morinda umbellata) belonging to the family Rubiaceae, using Gas Chromatography- Mass Spectrometry (GC-MS) analysis. Gynochthodes umbellata is an important medicinal plant widely used in traditional system of medicine. Literature survey revealed that till date, no work has been reported on GC-MS analysis of ethanol extract of Gynochthodes umbellata leaves. The mass spectrum of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of 32 compounds. Sterols, stanols and Terpenoids were the most prominent compounds detected in leaf ethanolic extract of this plant. The major compounds detected were 9,19-Cyclolanost-24-en-3-ol (16.09%), 2,5,5-Triphenyl-4-methoxyimidazole (10.87%), β-Sitosterol (8.81%), Stigmast-4-en-3-one (7.26%), Phytol (5.62%). The compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra.
S. Anjusha, A. Gangaprasad * and P. M. Radhamany
Plant Tissue Culture and Molecular Biology Lab, Department of Botany, University of Kerala, Thiruvananthapuram, Kerala - 695581, India
21 April, 2015
29 June, 2015
28 August, 2015
01 November, 2015